Ⅰ型膠原(COL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Collagen Type I (COL1)
- 編號USEA571Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.625-40ng/mL
- 靈敏度最小可檢測劑量小于等于0.224ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Ⅰ型膠原(COL1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型膠原(COL1)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-105 | 98 |
| EDTA plasma(n=5) | 87-104 | 96 |
| heparin plasma(n=5) | 81-98 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型膠原(COL1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅰ型膠原(COL1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 88-97% | 94-102% | 84-103% |
| EDTA plasma(n=5) | 95-103% | 99-105% | 87-95% | 91-104% |
| heparin plasma(n=5) | 80-97% | 88-103% | 80-99% | 88-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將Ⅰ型膠原(COL1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的Ⅰ型膠原(COL1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Ⅰ型膠原(COL1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Ⅰ型膠原(COL1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPA571Ra01 | Ⅰ型膠原(COL1)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA571Ra01 | Ⅰ型膠原(COL1)多克隆抗體 | WB; IHC |
| UPAA571Ra02 | Ⅰ型膠原(COL1)多克隆抗體 | WB |
| ULAA571Ra71 | Ⅰ型膠原(COL1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA571Ra | Ⅰ型膠原(COL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA571Ra | Ⅰ型膠原(COL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of hypertension | Urotensin II induces phenotypic differentiation, migration, and collagen synthesis of adventitial fibroblasts from rat aorta [PubMed: 18475149] |
| Cardiovascular Research | Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway [OxfordJournals: source] |
| Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa [PubMed: 19958244] |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source] |
| Connect Tissue Res. | Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans [Pubmed: 23758268] |
| Bulletin of Experimental Biology and Medicine | Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty [Pubmed: 24319714] |
| Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency [Springer: Source] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats [Pubmed: 24077847] |
| Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
| Physiol. Res. | The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818] |
| J Physiol Pharmacol. | Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet [Pubmed: 23568967] |
| 22 | Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses [ScienceDirect: S0142961214003135] |
| Journal of Clinical and Experimental Hepatology | Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis [ScienceDirect: S0973688314000073] |
| Human Molecular Genetics Online | Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10 [Pubmed:24659498] |
| PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
| Journal of Biomedical Materials Research Part A | Carboxymethyl cellulose—hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications [Pubmed:23720392] |
| Experimental Biology adn Medicine | Endostar, a novel human recombinant endostatin, attenuates liver fibrosis in CCl4-induced mice [Pubmed:24872431] |
| International Journal of Clinical & Experimental Pathology | Mast cell chymase in keloid induces profibrotic response via transforming growth factor-β1/Smad activation in keloid fibroblasts [Pubmed:25120737] |
| FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352] |
| Cardiology | KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload [Pubmed:25633973] |
| PLOS ONE | Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells [Pubmed:25635832] |
| Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198] |
| Macromolecular Bioscience | Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells [PubMed: 26126665] |
| journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite [Article: Jhtb] |
| Mol Med Rep | NIM811 downregulates transforming growth factor [PubMed: 26573209] |
| Front Pharmacol | Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats [PubMed: 26483688] |
| Chemosphere | Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue [PubMed: 25655816] |
| J Surg Res | Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition [PubMed: 25911951] |
| Molecular and Cellular Biochemistry | SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis [PubMed: 26112906] |
| Biointerphases | Incorporation of nanostructured hydroxyapatite and poly(N-isopropylacrylamide) in demineralized bone matrix enhances osteoblast and human mesenchymal stem cell activity [PubMed: 26443012] |
| Gels | On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery [2310-2861: 1] |
| Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470] |
| Int J Clin Exp Med | Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … [PubMed: 26309506] |
| International Journal of Molecular Sciences | Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration [Pubmed:27092492] |
| 中國眼耳鼻喉科雜志 | 白細(xì)胞介素10 上調(diào)表達(dá)對家兔慢性細(xì)菌性鼻竇炎黏膜創(chuàng)傷修復(fù)的影響 [article:11593] |
| Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470] |
| scientific reports | IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy [Pubmed:27263531] |
| Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496] |
| Кард?олог?я | ?нфаркт м?окарда, ускладнений аневризмою л?вого шлуночка: кл?н?чн? ? структурно-функц?ональн? особливост? переб?гу, д?агностичн?, терапевтичн? та прогностичн? аспекти [bitstream:123456789] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Indian Journal of Pharmaceutical Education and Research | Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-β1 [sites:default] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| biochemical and biophysical research communications | Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. [pubmed:28153731] |
| Frontiers in Pharmacology | Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-β1/Smad-mediated epithelial to mesenchymal … [PMC5108931] |
| International?Journal?of?Molecular?Sciences | Plasma?LncRNA-ATB, a?Potential?Biomarker?for?Diagnosis?of?Patients?with?Coal?Workers'Pneumoconiosis: A?Case-Control?Study. [pubmed:27556453] |
| BMC Cancer | Saikosaponin?d?induces?cell?death?through?caspase-3-dependent,?caspase-3-independent?andmitochondrial?pathways?in?mammalian?hepatic?stellate?cells. [pubmed:27461108] |
| J Cell Physiol. | An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. [pubmed:28240358] |
| Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440] |
| Acta Biomater. | Antiresorptive and anti-angiogenetic octacalcium phosphate functionalized with bisphosphonates: An in vitro tri-culture study. [pubmed:28238916] |
| The American Journal of Chinese Medicine | Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells [pubmed:28231747] |
| Biomedical and Environmental Sciences | Crystalline Silica Promotes Rat Fibrocyte Differentiation?in Vitro, and Fibrocytes Participate in Silicosis?in Vivo [pubmed:29081339] |
| Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111] |
| Dental Materials Journal | Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells. [pubmed:28228629] |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995] |
| Journal of Applied Biomaterials & Functional Materials | Demineralized dentin and enamel matrices as suitable substrates for bone regeneration [pubmed:28731486] |
| Acta Biomaterialia | Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation [pubmed:28698163] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| Macromolecular?Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029] |
| International Journal of Molecular Medicine | Adenovirus?mediated knockdown of activin A receptor type 2A attenuates immune?induced hepatic fibrosis in mice and inhibits interleukin?17?induced?… [Pubmed:29620144] |
| PLoS One | Effect of hyperbaric oxygen therapy (HBO) on implant-associated osteitis in a femur fracture model in mice [Pubmed:29377928] |
| Royal?Society?of?Chemistry | In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment [c8ra00768c] |
| European Food Research and Technology | Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis [10.1007:s00217-018-3119-z] |
| Journal of?Applied?Biomaterials?&?Functional?Materials | BMP-2 and type I collagen preservation in human deciduous teeth after demineralization [Pubmed:30045659] |
| Theriogenology | Effect of transforming growth factor-β1 on α-smooth muscle actin and collagen expression in equine endometrial fibroblasts [Pubmed: 30321755] |
| ACS?Applied?Materials?&?Interfaces | Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications [Pubmed: 30433758] |
| Chemico-Biological?Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells [Pubmed: 30731080] |
| Oxidative Medicine and Cellular Longevity | Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway [] |
| Tropical Journal of Pharmaceutical Research | Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats. [] |
| Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
| Experimental Lung Research | High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects [Pubmed: 31290711] |
| BMC Nephrology | Serum sclerostin levels are positively related to bone mineral density in peritoneal dialysis patients: a cross-sectional study [Pubmed: 31315601] |
| Archives of Dermatological Research | Extracellular matrix deposition by adipose-derived stem cells and fibroblasts: a comparative study [Pubmed: 31616972] |
| Drug Delivery and Translational Research | A multifunctional dexamethasone-delivery implant fabricated using atmospheric plasma and its effects on apoptosis, osteogenesis and inflammation [Pubmed: 31898081] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs [Pubmed: 32146192] |
| Molecular Metabolism | SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α [Pubmed: 32029232] |
| ACS Applied Materials & Interfaces | ZIF-8 Modified Multifunctional Bone-Adhesive Hydrogels Promoting Angiogenesis and Osteogenesis for Bone Regeneration [Pubmed: 32814397] |
| in vitro cellular & developmental biology-animal | Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix [Pubmed: 32588253] |
| Molecular Medicine | Circulating fibrocytes traffic to the lung in murine acute lung injury and predict outcomes in human acute respiratory distress syndrome: a pilot study [Pubmed: 32460694] |
| Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction [] | |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
| Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда [] | |
| Ann Med Surg | Trauma magnitude of the meta-epyphyseal cancellous affects the incidence of deep vein thrombosis. A prospective cohort study on the dynamic of collagen I ?- [33717478] |
| INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Uncarboxylated osteocalcin reverses the high glucose?induced inhibition of the osteogenic differentiation of MC3T3E1 cells via the GPRC6A/cAMP/PKA/AMPK signaling pathway [33786607] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells [] | |
| Russian Journal of Cardiology | Early diagnosis of myocardial fibrosis in patients with epicardial obesity [] |
| Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction [] | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate?… [34857049] |
| Pharmacol Res | Esomeprazole Alleviates Fibrosis in Systemic Sclerosis by Modulating AhR/Smad2/3 Signaling [Pubmed:34995795] |
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