白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interleukin 8 (IL8)
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1
- 編號USEA080Ga
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.8pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1277 ¥ 1824 ¥ 8208 ¥ 15504 ¥ 127680
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測白介素8(IL8)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素8(IL8)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-102 | 96 |
| EDTA plasma(n=5) | 89-98 | 92 |
| heparin plasma(n=5) | 79-97 | 80 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素8(IL8)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素8(IL8)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-99% | 87-98% | 91-101% | 84-101% |
| EDTA plasma(n=5) | 93-105% | 96-103% | 79-89% | 82-94% |
| heparin plasma(n=5) | 84-96% | 91-105% | 79-102% | 87-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將白介素8(IL8)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的白介素8(IL8)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素8(IL8)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的白介素8(IL8)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA080Ga01 | 白介素8(IL8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA080Ga01 | 白介素8(IL8)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA080Ga01 | 白介素8(IL8)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA080Ga71 | 白介素8(IL8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA080Ga81 | 白介素8(IL8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA080Ga22 | 白介素8(IL8)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA080Ga21 | 白介素8(IL8)單克隆抗體 | ICC/IF |
| UMAA080Ga23 | 白介素8(IL8)單克隆抗體 | ICC/IF |
| USEA080Ga | 白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA080Ga | 白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA080Ga | 白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA080Ga01 | 白介素8(IL8)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA080Ga01 | 白介素8(IL8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Reproductive Biology and Endocrinology | Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow [Rbej: 14777827] |
| Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa [PubMed: 19958244] |
| Journal of Clinical Periodontology | Experimental peri-implant mucositis at different implant surfaces. [Pubmed: 24521508] |
| Physiological Research | Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. [Pubmed: 2432969] |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186] |
| J Med Life. | Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination [Pubmed:Pmc4197484] |
| J Clin Periodontol. | Experimental peri‐implant mucositis at different implant surfaces [Pubmed:24521508] |
| British Journal of Nutrition | Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers [Pubmed:24846452] |
| Theriogenology | Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis [Theriojournal:Source] |
| Anim Reprod Sci. | Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes [PubMed: 25851495] |
| Медицина и здравоохранение | ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕ [Article: N] |
| BMC Anesthesiology | Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning [PubMed: 25943099] |
| J Breath Res | Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery [PubMed: 25719610] |
| ARTICLE | The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt [Profile: Mohmad_Mosaad] |
| Veterinary Immunology and Immunopathology | Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period [Pubmed:27090626] |
| Infection, Genetics and Evolution | Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression [Pubmed:27349993] |
| Asian Pacific Journal of Tropical Medicine | Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury [science:S1995764516300955] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu] |
| Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610] |
| journal of molecular and cellular cardiology | Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. [pubmed:28088561] |
| Physiological Research | Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury. [pubmed:28006948] |
| PLOS?ONE | Mechanical?Ventilation?Alters?the?Development?of?Staphylococcus?aureus?Pneumonia?in?Rabbit. [pubmed:27391952] |
| The Journal of Comenius University in Bratislava | Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury [acm.2017.16.issue-3] |
| Journal of Physiology and Pharmacology | Effects?of?budesonide?on the?lung?functions,?inflammation?and?apoptosis?in a?saline-lavagemodel?of?acute?lung?injury. [pubmed:28195073] |
| 中國藥物警戒 | 小劑量阿奇霉素對穩(wěn)定期老年慢性阻塞性肺疾病患者外周血內(nèi)TNF-α、IL-8、CRP水平及肺功能的影響 [Periodical_zgywjj201608004] |
| Veterinary Immunology and Immunopathology | Cytokine levels in colostrum and in foals' serum pre- and post-suckling [pubmed:28242000] |
| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057] |
| European Journal of Pharmacology | Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study [pubmed:28684235] |
| PLoS One | Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation [pubmed:29149185] |
| Archives of Oral Biology | The effect of restorative materials on cytokines in gingival crevicular fluid [pubmed:28992599] |
| Experimentelle periimplant?re Mukositis an unterschiedlichen Implantatoberfl?chen [doi: 10.1111/jcpe.12240.] | |
| Molecular?Medicine?Reports | CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB [pubmed:28627614] |
| ARTIFICIAL?CELLS?NANOMEDICINE AND BIOTECHNOLOGY? | Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis [pubmed:28608724] |
| Biological Rhythm Research | Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows [10.1080/09291016.2017.1319636] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665] |
| Journal of Ultrasound in Medicine | Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells. [pubmed:28600899] |
| Veterinary Immunology and Immunopathology | Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. [pubmed:28895861] |
| Biomedicine hub | Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits [10.1159/000475877] |
| Anticancer Research | Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab. [pubmed:28982871] |
| Chronic Respiratory Disease | Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort. [pubmed:29117798] |
| Inflammopharmacology | Polar extract of protects cartilage homeostasis: possible mechanism of action [Pubmed:29313174] |
| Life?Sciences | Dexmedetomidine attenuates renal fibrosis via α2-adrenergic receptor-dependent inhibition of cellular senescence after renal ischemia/reperfusion [Pubmed:29729264] |
| Microbial Pathogenesis | Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos?… [Pubmed:29787791] |
| Lasers in Medical Science | Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears [Pubmed:30003425] |
| Medical Science Monitor | Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)?… [Pubmed:29804125] |
| Journal of Veterinary?Science | Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study [Pubmed:29984902] |
| Bioscience Reports | N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. [Doi: 10.1042/BSR20180858] |
| Acta?Medica?Mediterranea | Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung?… [Doi: 10.1515/acm-2016-0012] |
| Journal of?Dairy?Science | Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows [Pubmed: 17430930] |
| Biomedicine?&?Pharmacotherapy | Interleukin 8 targeted contrast echocardiography is effective to evaluate myocardial ischemia-reperfusion injury in the rabbits [] |
| Emerging?Microbes?&?Infections | Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin [Pubmed: 30405111] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| International Journal of Chronic Obstructive Pulmonary Disease | Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD [Pubmed: 30655663] |
| Inflammopharmacology | Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard & [] |
| Journal of Hematology &?Oncology | Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment?… [Pubmed: 30621731] |
| BioMed Research International | Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts [] |
| Frontiers in Pharmacology | Naoxintong Capsule Inhibits the Development of Cardiovascular Pathological Changes in Bama Minipig Through Improving Gut Microbiota [Pubmed: 31632272] |
| international journal of molecular sciences | Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764] |
| Scientific Reports | Corrosion Resistance of Graphene oxide/Silver Coatings on Ni–Ti alloy and Expression of IL-6 and IL-8 in Human Oral Fibroblasts [Pubmed: 32094428] |
| Journal of Receptors and Signal Transduction Research | Suppressor of ras val-2 promotes inflammation-mediated oxidative stress and cell apoptosis in cardiomyocytes through activating Mst1-mROS signaling pathway [Pubmed: 32065019] |
| Antibiotics | Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs [Pubmed: 31963653] |
| Physiol Res | Effect of Different Dosages of Dexamethasone Therapy on Lung Function and Inflammation in an Early Phase of Acute Respiratory Distress Syndrome Model [Pubmed: 31928043] |
| Respiratory Research | Synthetic surfactant with a recombinant surfactant protein C analogue improves lung function and attenuates inflammation in a model of acute respiratory?… [Pubmed: 31694668] |
| Journal of Pharmacy and Pharmacology | Tectorigenin inhibits inflammation and pulmonary fibrosis in allergic asthma model of ovalbumin‐sensitized guinea pigs [Pubmed: 32314371] |
| Inflammation | Cyanidin-3-O-Glucoside Attenuates Lipopolysaccharide-Induced Inflammation in Human Corneal Epithelial Cells by Inducing Let-7b-5p-Mediated HMGA2/PI3K/Akt?… [Pubmed: 32248330] |
| Sci Rep | NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population [Pubmed: 33177541] |
| International Journal of Nanomedicine | Study on Adenovirus Infection in vitro with Nanoself-Assembling Peptide as Scaffolds for 3D Culture [Pubmed: 32922004] |
| Theriogenology | A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows [Pubmed: 32810791] |
| Cell Cycle | SIRT1 relieves Necrotizing Enterocolitis through inactivation of Hypoxia-inducible factor (HIF)-1a [Pubmed: 32657204] |
| Frontiers in Pharmacology | Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy [Pubmed: 32508661] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?… [] |
| Animals | Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … [] |
| INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis [] |
| HMGB1 e citocinas inflamatórias em les?o pulmonar aguda experimental induzida em coelhos [] | |
| Bull Exp Biol Med | Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors [33222085] |
| physiological reports | Impact of synthetic surfactant CHF5633 with SP?\B and SP?\C analogues on lung function and inflammation in rabbit model of acute respiratory distress syndrome [33403805] |
| J Med Food | Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs [33861937] |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223] |
| Sci Rep | Corrosion Resistance of Graphene oxide/Silver Coatings on Ni¨CTi alloy and Expression of IL-6 and IL-8 in Human O... [32094428] |
| Poultry Science | Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine??- [33518110] |
| Bioactive Materials | Construction of heparin-based hydrogel incorporated with Cu5. 4O ultrasmall nanozymes for wound healing and inflammation inhibition [33778192] |
| animals | Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic??- [34066204] |
| Sci Rep | Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749] |
| Pharmaceutics | Nitric-Oxide-Releasing Dexamethasone Derivative NCX-1005 Improves Lung Function and Attenuates Inflammation in Experimental Lavage-Induced ARDS [34959373] |
| Biomedicines | Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes [34829910] |
| Anesthesiology | Adverse Mechanical Ventilation and Pneumococcal Pneumonia Induce Immune and Mitochondrial Dysfunctions Mitigated by Mesenchymal Stem Cells in Rabbits [Pubmed:34965287] |
| J Clin Med | Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study [Pubmed:35566605] |
| Microb Pathog | Cell damage and neutrophils promote the infection of Mycoplasma pneumoniae and inflammatory response [Pubmed:35724831] |
| ResearchSquare | LncRNA KCNQ10T1 shuttled by bone marrow mesenchymal stem cells-derived exosome inhibits sepsis via regulation of miR-154-3p/RNF19A axis [] |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS [] | |
| Intensive Care Research | Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD [] |
留言咨詢
