18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Cathelicidin Antimicrobial Peptide (CAMP)
CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
- 編號UCEC419Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍24.69-2000ng/mL
- 靈敏度最小可檢測劑量小于等于9.16ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1466 ¥ 2094 ¥ 9423 ¥ 17799 ¥ 146580
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測18kDa陽離子抗菌肽(CAMP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的18kDa陽離子抗菌肽(CAMP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-104 | 89 |
| EDTA plasma(n=5) | 79-92 | 84 |
| heparin plasma(n=5) | 79-89 | 81 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的18kDa陽離子抗菌肽(CAMP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中18kDa陽離子抗菌肽(CAMP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 81-91% | 78-90% | 91-98% |
| EDTA plasma(n=5) | 80-94% | 84-93% | 93-101% | 87-101% |
| heparin plasma(n=5) | 79-94% | 86-102% | 83-104% | 89-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將18kDa陽離子抗菌肽(CAMP)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPC419Ra01 | 18kDa陽離子抗菌肽(CAMP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC419Ra01 | 18kDa陽離子抗菌肽(CAMP)多克隆抗體 | WB |
| USEC419Ra | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEC419Ra | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC419Ra | 18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC419Ra11 | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Urology | Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells [Pubmed:25681250] |
| Urology | Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells [PubMed: 25681250] |
| Sarcoidosis Vasc Diffuse Lung Dis | Cathelicidin as a link between sarcoidosis and tuberculosis [PubMed: 26422567] |
| Allergy and Asthma Proceedings | The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children [PubMed: 26108071] |
| J Clin Microbiol. | Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis. [Pubmed:27008883] |
| experimental dermatology | Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin. [pubmed:27315464] |
| PLOS?ONE | Reduced?Expression?of the?Extracellular?Calcium-Sensing?Receptor?(CaSR) Is?Associated?withActivation?of the?Renin-Angiotensin?System?(RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. [pubmed:27391973] |
| Front Pharmacol.? | Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. [pubmed:28261091] |
| Allergologia et immunopathologia | The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin [pubmed:27955890] |
| Chinese Journal of Osteoporosis and Bone Mineral Research | Effects of cold exposure on the bone marrow adipose tissue [DOI: 10.3969/j.issn.1674-2591.2017.05.008] |
| Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique [Pubmed:29374283] |
| Experimental Parasitology | Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin [Pubmed: 30904694] |
| Theranostics | Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling [Pubmed: 31149039] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37 [Pubmed: 32050475] |
| MICROB PATHOGENESIS | Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy [Pubmed: 32645421] |
| International Journal of Nanomedicine | Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide [Pubmed: 32606669] |
| 博士論文 | Rela??es causais entre a express?o dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com?… [] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| New Med | Analysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between??- [] |
| Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea [] | |
| Int J Mol Sci | Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection [Pubmed:35328414] |
| Cell Rep | LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity [Pubmed:35649354] |
