雌二醇(E2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Estradiol (E2)
17B-Estradiol; Oestradiol; Beta-Estradiol
- 編號UKSA461Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.45pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 2149 ¥ 3582 ¥ 6448 ¥ 12537 ¥ 21492
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對雌二醇(E2)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA461Ge11 | 雌二醇(E2)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA461Ge21 | 雌二醇(E2)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA461Ge31 | 雌二醇(E2)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA461Ge01 | 雌二醇(E2)多克隆抗體 | ELISA |
| ULAA461Ge71 | 雌二醇(E2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA461Ge81 | 雌二醇(E2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA461Ge26 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA461Ge21 | 雌二醇(E2)單克隆抗體 | IHC |
| UMAA461Ge22 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA461Ge29 | 雌二醇(E2)單克隆抗體 | ELISA |
| UMAA461Ge28 | 雌二醇(E2)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA461Ge27 | 雌二醇(E2)單克隆抗體 | ELISA |
| UCEA461Ge | 雌二醇(E2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA461Ge | 雌二醇(E2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA461Ge | 雌二醇(E2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA461Ge | 雌二醇(E2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA461Ge | 雌二醇(E2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA461Ge11 | 雌二醇(E2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| BioMed research international | Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced Osteoporosis [PubMed: PMC3678416] |
| Fertility and Sterility | Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356] |
| Repositório Digital | Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongas [Lume: Source] |
| Electromagnetic Biology and Medicine | The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats [Informahealthcare: Source] |
| Academic Sciences | Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats [Ijppsjournal: Source] |
| Stem Cells Int. | Cell Therapy for Chemically Induced Ovarian Failure in Mice [Pubmed:Pmc4274854] |
| Reproductive Toxicology | Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring [Pubmed:24126190] |
| Phytomedicine. | Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice [Pubmed:24932975] |
| Turkish Journal of Urology | Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source] |
| international journal of environmental research and public health | Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886] |
| Reprod Toxicol | In utero [PubMed: 25530038] |
| oncology research | Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats [PubMed: 26629940] |
| Environ Toxicol | Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period [PubMed: 25782373] |
| J Endocrinol | Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction [PubMed: 25770118] |
| Reproduction | Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats [PubMed: 26556892] |
| Veterinary Word | Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212] |
| Archives of Physiology and Biochemistry | Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder [Pubmed:26934364] |
| Plos One | Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642] |
| Biology of Reproduction | Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring [Pubmed:26962114] |
| PPAR Research | Testosterone?Replacement?Modulates?Cardiac?Metabolic?Remodeling?after?Myocardial?Infarction?byUpreCavia (Guinea pig )lating?PPARα [Pubmed:27413362] |
| Clinical and Experimental Hypertension | Assessment of ADMA, estradiol, and progesterone in severe preeclampsia [Pubmed:27152507] |
| PLoS Genet. | A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis [Pubmed:26977770] |
| Scientific Reports | The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization [pubmed:27876896] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
| African Journal of Environmental Science and Technology | Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania [150659] |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women [S1756464617301561] |
| environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061] |
| Environmental toxicology and pharmacology | Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. [pubmed:28376377] |
| PPAR?Research | Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα [pubmed:27413362] |
| Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
| Interact Cardiovasc Thorac Surg | Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats [pubmed:29049608] |
| Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
| Reproductive Sciences | Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome [pubmed:28673204] |
| Oncotarget | Rcan2 and estradiol independently regulate body weight in female mice [pubmed:28624805] |
| African Journal of Environmental Science and Technology | Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania [10.5897/AJEST2016.2234] |
| Journal of Orthopaedic Research | The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice [pubmed:29058779] |
| Animal Production Science | Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? [10.1071/ANv57n12Ab065] |
| Asian Journal of Biomedical and Pharmaceutical Sciences | A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/???2-catenin Signaling Pathways in Ovariectomized Rats [ISSN 2249 – 622X] |
| Nanotheranostics | The biocompatibility studies of polymer dots on pregnant mice and fetuses [pubmed:29071192] |
| DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999] |
| Reproduction. | Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring [pubmed:28687593] |
| Molecular Medicine Reports | Molecular mechanism and functional role of macrophage colony?stimulating factor in follicular granulosa cells [pubmed:28656272] |
| Annals of Rheumatic Diseases? | FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study [Suppl_2:728.1.abstract] |
| Stem?Cell?Research?&?Therapy | The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Aquatic Toxicology | Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations [Pubmed:29775925] |
| Journal of Ethnopharmacology | Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model [Pubmed:29705515] |
| Inflammation | Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male [Pubmed:29737476] |
| European?Journal of Neuroscience | Postnatal separation prevents the development of prenatal stress‐induced anxiety in association with changes in oestrogen receptor and oxytocin immunoreactivity in?… [Pubmed:29205599] |
| Journal of?Ovarian?Research | DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035] |
| Medical?Science?Monitor | Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian?… [Pubmed:29674607] |
| Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
| Stem?Cell?Research?&?Therapy | Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the?… [Pubmed: 30606243] |
| journal of molecular neuroscience | Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical Approaches [Pubmed: 30600432] |
| Heliyon | Establishment and evaluation of a novel mouse model of peri/postmenopausal depression [Doi: 10.1016/j.heliyon.2019.e01195] |
| Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol?induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary [] |
| Evidence-Based Complementary and Alternative Medicine | iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine [] |
| Biomedicine & Pharmacotherapy | G protein-coupled estrogen receptor activation improves contractile and diastolic functions in rat renal interlobular artery to protect against renal ischemia?… [Pubmed: 30784936] |
| Biomedicine & Pharmacotherapy | Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve [] |
| Biology of Reproduction | G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide?… [Pubmed: 31034039] |
| Biomedicine & pharmacotherapy | Aqueous extract of lily bulb ameliorates menopause-like behavior in ovariectomized mice with novel brain-uterus mechanisms distinct from estrogen therapy [Pubmed: 31207575] |
| Reproductive Biology and Endocrinology | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in?… [Pubmed: 31311554] |
| Experimental Neurology | Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause [Pubmed: 31377402] |
| Oxidative medicine and cellular longevity | Total Polysaccharides of Lily Bulb Ameliorate Menopause-Like Behavior in Ovariectomized Mice: Multiple Mechanisms Distinct from Estrogen Therapy [Pubmed: 31428228] |
| Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718] |
| Cellular Signalling | Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitro [Pubmed: 31704004] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
| Neuroscience | Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female Rats [Pubmed: 31805254] |
| Open Access Maced J Med Sc | Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats-Model Gastritis [Pubmed: 31949507] |
| Estradiol treatment during perinatal development alters [Pubmed: 31966342] | |
| science of the total environment | Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells [Pubmed: 32334352] |
| molecular nutrition & food research | Lepidiline A Improves the Balance of Endogenous Sex Hormones and Increases Fecundity by Targeting HSD17B1 [Pubmed: 32239630] |
| Chemosphere | Prenatal exposure of female mice to per?uorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21?… [Pubmed: 33131727] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) [Pubmed: 33011509] |
| Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084] |
| JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive rats [Pubmed: 32790534] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
| Antioxidants (Basel) | Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model [Pubmed: 32517356] |
| Andrologia | Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty [Pubmed: 32478927] |
| Neurotoxicology | Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice [Pubmed: 32407858] |
| archives of toxicology | Effect of estrogen-active compounds on the expression of RACK1 and immunological implications [Pubmed: 32328699] |
| Heliyon | Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting?… [Pubmed: 33005806] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
| biorxiv | The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice [] |
| Moosense pedometer activity and periestrual hormone profile in relation to oestrus in crossbred cattle [] | |
| J Steroid Biochem Mol Biol | Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development [33465420] |
| JOURNAL OF ETHNOPHARMACOLOGY | Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655] |
| JOURNAL OF ETHNOPHARMACOLOGY | BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-|êB pathways in aging mice induced by D-galactose [33508368] |
| Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice [] | |
| Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency [] | |
| Arch Gynecol Obstet | The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese?… [34417839] |
| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391] |
| Stem Cells Int | Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in?… [34733331] |
| Eur J Pharmacol | 17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice [34822791] |
| IBRO Neuroscience Reports | Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice [34746914] |
| Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459] |
| Nat Commun | TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice [Pubmed:35177657] |
| Appl Biochem Biotechnol | ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary [Pubmed:35138554] |
| Stem Cell Research & Therapy | G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and?… [] |
| Research Square | Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency [] |
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