基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Matrix Metalloproteinase 1 (MMP1)
CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
- 編號USCA097Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍2.74-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于1.17pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1915 ¥ 2736 ¥ 12312 ¥ 23256 ¥ 191520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測基質(zhì)金屬蛋白酶1(MMP1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的基質(zhì)金屬蛋白酶1(MMP1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 95 |
| EDTA plasma(n=5) | 84-99 | 90 |
| heparin plasma(n=5) | 87-95 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)金屬蛋白酶1(MMP1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中基質(zhì)金屬蛋白酶1(MMP1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-88% | 88-96% | 88-96% | 92-105% |
| EDTA plasma(n=5) | 80-101% | 79-103% | 89-103% | 81-90% |
| heparin plasma(n=5) | 89-96% | 95-104% | 88-95% | 93-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將基質(zhì)金屬蛋白酶1(MMP1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的基質(zhì)金屬蛋白酶1(MMP1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的基質(zhì)金屬蛋白酶1(MMP1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的基質(zhì)金屬蛋白酶1(MMP1)呈正相關(guān)。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA097Rb01 | 基質(zhì)金屬蛋白酶1(MMP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA097Rb51 | 基質(zhì)金屬蛋白酶1(MMP1)多克隆抗體 | WB |
| UMAA097Rb21 | 基質(zhì)金屬蛋白酶1(MMP1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA097Rb | 基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA097Rb | 基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA097Rb | 基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA097Rb | 基質(zhì)金屬蛋白酶1(MMP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| The Journal of Pathology | Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation [Pubmed:25186281] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
| J Immunol | Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis [PubMed: 26268658] |
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| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
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| Journal of Cellular Physiology | Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057] |
| Journal of Applied Animal Research | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208] |
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| Journal of Agricultural and?Food?Chemistry | Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice [Pubmed:30032605] |
| Oral?Diseases? | TIMP‐1 association with collagen type I overproduction in hereditary gingival fibromatosis [Pubmed:29989318] |
| Journal of Applied Animal Research? | Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis [10.1080:09712119.2017.1335208] |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166] |
| МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ [] | |
| International Journal of Molecular Sciences | A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor [Pubmed: 31052424] |
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| Journal of Plastic, Reconstructive & Aesthetic Surgery | Intraluminal fluid infusion in a rat jejunum ischemia/reperfusion model is associated with improved tissue perfusion and less mucosal damage [Pubmed: 31734236] |
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| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
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| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes [Pubmed:35340587] |
