腫瘤蛋白p53(P53)多克隆抗體
Polyclonal Antibody to Tumor Protein p53 (P53)
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- 編號UPAA928Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA928Hu01-腫瘤蛋白p53(P53)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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DAB staining on IHC-P;<br/>Sample: Porcine Colon Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Porcine Lymph node Tissue<br/>Primary Ab: 10μg/ml Rabbit Anti-Human P53 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Figure 1. Western Blot; Sample: Recombinant TP53, Human.
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Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Human 293T cell lysate<br/>Primary Ab: 1μg/mL Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Knockout Varification: <br/>Lane 1: Wild-type MCF7 cell lysate; <br/>Lane 2: TP53 knockout MCF7 cell lysate; <br/>Predicted MW: 24,30,33,34,38,39,44kDa <br/>Observed MW: 50kDa<br/>Primary Ab: 1μg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate<br/>Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Sample: Porcine Skin lysate<br/>Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對P53的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別P53。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA928Hu01 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA928Hu02 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA928Hu03 | 腫瘤蛋白p53(P53)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA928Hu01 | 腫瘤蛋白p53(P53)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA928Hu01 | 腫瘤蛋白p53(P53)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA928Hu02 | 腫瘤蛋白p53(P53)多克隆抗體 | WB; IHC; FCM |
| ULAA928Hu71 | 腫瘤蛋白p53(P53)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA928Hu22 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| UMAA928Hu24 | 腫瘤蛋白p53(P53)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA928Hu25 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| UMAA928Hu27 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| UMAA928Hu21 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| UMAA928Hu23 | 腫瘤蛋白p53(P53)單克隆抗體 | WB |
| UFAA928Hu04 | 抗腫瘤蛋白p53(P53)單克隆抗體 | FCM |
| UFAA928Hu02 | 抗腫瘤蛋白p53(P53)單克隆抗體 | FCM |
| USEA928Hu | 腫瘤蛋白p53(P53)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA928Hu | 腫瘤蛋白p53(P53)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA928Hu | 腫瘤蛋白p53(P53)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA928Hu01 | 腫瘤蛋白p53(P53)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233] |
| Microscopy Research and Technique | Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde [PubMed: 25761397] |
| J Nutr Biochem | Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice [PubMed: 26344777] |
| Biochemical and Biophysical Research Communications | Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes [Pubmed: 31331641] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats [] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] |
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