脂質(zhì)運載蛋白4(LCN4)活性蛋白
Active Lipocalin 4 (LCN4)
VNSP2; Vomeronasal secretory protein II
- 編號UAPS456Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點5.9
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 暫無報價 暫無報價 暫無報價 暫無報價 暫無報價
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活性實驗
Interleukin 33 (IL33) is a dual-function protein that acts both as a traditional cytokine and an intracellular nuclear factor. It is primarily released by endothelial and epithelial cells upon cellular damage or stress, serving as an "alarmin" to alert the immune system. IL33 signals through its receptor ST2 to drive type 2 immune responses, which are crucial in allergic inflammation, host defense against parasites, and tissue repair.Besides,Thymic Stromal Lymphopoietin (TSLP) has been identified as an interactor of IL33, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IL33 and recombinant human TSLP. Briefly, IL33 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TSLP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL33 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse IL33 and recombinant human TSLP was shown in Figure 1, the EC50?for this effect is 0.23 μg/mL..
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
| UAPS456Mu01 | 脂質(zhì)運載蛋白4(LCN4)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPS456Mu01 | 脂質(zhì)運載蛋白4(LCN4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAS456Mu01 | 脂質(zhì)運載蛋白4(LCN4)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAS456Mu81 | 脂質(zhì)運載蛋白4(LCN4)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAS456Mu71 | 脂質(zhì)運載蛋白4(LCN4)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USES456Mu | 脂質(zhì)運載蛋白4(LCN4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |

